The human promyelocytic leukemia cell line HL-60 overexpresses the c-myc protooncogene. A calculated secondary structure for c-myc mRNA placed the initiation codon in a bulge of a weakly base-paired region. Treatment of HL-60 cells with 5' d (AACGTTGAGGGGCAT) 3', complementary to the initiation codon and the next four codons of c-myc mRNA, inhibited c-myc protein expression in a dose … Cell line and culture. Human HL-60 cells were purchased from the Shanghai Institute for Biological Science and maintained in IMDM (Gibco, MD, USA) supplemented with 20% fetal bovine serum (FBS; Gibco, MD, USA) in an environment with 5% CO 2 at 37 … The NCI-60 cancer cell line panel is a group of 60 human cancer cell lines used by the National Cancer Institute (NCI) for the screening of compounds to detect potential anticancer activity. Similar results were obtained when the cells were pretreated with Ru360, a specific blocker of Ca2+ uptake into mitochondria. The findings suggest that H2O2-induced caspase-3 and -9 activation and mitochondrial membrane depolarization is dependent on rises in [Ca2+]c in human myeloid HL-60 cells. HL-60 cells adhered to the HS-5 Bone-Marrow Stromal-Cell (BMSC) layer via pseudopodia. To determine the influence of HS-5 cells on the survival of HL-60 cells, the latter were cultured on HS-5 for 24, 48, 72, or 96 h, and the viability of the HL-60 cells was then determined by the Cholecystokinin-8 (CCK8) test. HL-60 cells were seed in different concentration VP for 24 h, and the DMSO treatment is control group. Cell viability assay. The Cell Counting Kit-8 (CCK-8) assay (7Sea Biotech, Shanghai, China) was used to test cell viability. Cells in each group (1x10 4 /well) were seeded in 96-well plates and incubated. In brief, 10 μL of CCK-8 (7Sea Cell The HL-60 cell line was derived from peripheral blood leukocytes obtained by leukopheresis of a 36-year-old Caucasian female with acute promyelocytic leukemia. It was among the first long-term suspension cultures of human myeloid leukaemic cells to be established. HL-60 cells were exposed to ultrasound for up to 3min in the presence and absence of AlPcTS. The samples treated with AlPcTS alone were maintained in the same position for the same period as cells receiving ultrasonic exposure. Ultrasonic output from the transducer was evaluated in degassed water by placing the axis of the transducer horizontally. We investigated the regulatory roles of USP2 in mRNA accumulation of proinflammatory cytokines in macrophage-like cells after stimulation with a toll-like receptor (TLR) 4 ligand, lipopolysaccharide (LPS). Human macrophage-like HL-60 cells, mouse macrophage-like J774.1 cells, and mouse peritoneal macrophages demonstrated negative feedback to USP2 mRNA levels after LPS stimulation, … Pre Caspase Cell Viability Cell viability was assessed by Trypan Blue Solution, 0.4% manual cell counting after treatment with different ginger concentrations for 8, 12 or 24 hours. Figure 3 displays the cell viability of HL 60 cells and data represents the mean percent viability from two independent experiments.
Beta-Lap induced apoptosis in HL-60 cells in a dose-dependent manner as measured by flow cytometry and DNA ladder formation. Cell cycle changes, such as accumulations in S and G2-phases, were not observed. Apoptosis was accompanied by activation of caspase 3 and concomitant cleavage of poly(ADP-ribose) polymerase (PARP) to an 89 kDa polypeptide
In HL-60 cells, BBR induced chromatin condensation and DNA fragmentation, and triggered the activation of PARP, caspase-3 and caspase-8 without the activation of caspase-9. BBR-induced DNA fragmentation was abolished by pretreatment with inhibitors against caspase-3 and caspase-8, …
Question: You Want To Add PMA To 80 ML Of HL-60 Cells So That You Have A Concentration Of 10 ηM. Your Stock Solution Of PMA Is 5 M. What Is The Volume Of You Stock Solution You Need? This question hasn't been answered yet Ask an expert. An HL-60 cell variant resistant to induction of cell differentiation by phorbol diesters, designated as R-80, was isolated by culturing HL-60 cells for 80 subcul tures (5- to 8-day intervals) in the presence of increasing concentrations of PMA (Chemicals for Cancer Research, Eden Prairie, Minn.) (20, 22, 24, 36). (2003). γ-Irradiation Enhances Apoptosis Induced by Cannabidiol, a Non-psychotropic Cannabinoid, in Cultured HL-60 Myeloblastic Leukemia Cells. Leukemia & Lymphoma: Vol. 44, No. 10, pp. 1767-1773. Programmed Cell Death Induced by (−)-8,9-Dehydroneopeltolide in Human Promyelocytic Leukemia HL-60 Cells under Energy Stress Conditions. By Haruhiko Fuwa *, Mizuho Sato and Makoto Sasaki. Graduate School of Life Sciences, Tohoku University, 2-1-1 Katahira, Aoba-ku, Sendai 980-8577, Japan. *.
Ceramide, a sphingolipid (SL), plays a critical role in cancer cell apoptosis in response to chemotherapy. Here, we investigated the effects of chemotherapy selection pressure with Ara-C and dnr on SL composition and enzyme activity in the AML cell line HL-60. Description: The clone 15 HL-60 has been derived from HL-60 cells (ECACC catalogue no. 98070106) that had been cultured at an elevated pH of 7.6 - 7.8 for 2 months.Clone 15 HL-60 was picked from soft agar being selected for the ability to differentiate to eosinophils after treatment with butyric acid. Media in category "HL-60 cells" The following 13 files are in this category, out of 13 total. Play media. Adenosine-A3-receptors-in-neutrophil-microdomains-promote-the-formation-of-bacteria-tethering-embor201389s2.Ogv 6.6 s, 768 411 KB. Play media. The HL-60 cells constitutively express a high level of the H 1, H 2, and H 4 receptors (31, 32). These cells served as a positive control. The cells were cultured as previously described (25). Washed PMNs or HL-60 cells at 10 8 /ml were incubated for 30 min in PBS with either 1 mM sodium periodate (Sigma Chemical Co.) at 0 C or 10 mM sodium periodate at room temperature for oxidation of surface sialic acid or total carbohydrates, respectively. Reference: 1468 Shimizu N, Ohta M, Fujiwara C, Sagara J, Mochizuki N, Oda T, Utiyama H A Gene Coding for a Zinc Finger Protein Is Induced during 12-O-Tetradecanoylphorbol-13-Acetate-Stimulated HL-60 Cell Differentiation J Biochem 1992 111:272-277 PubMed ID: 1569051 : 5042 Haruta Y, Seon BK. HL‐60 cell differentiation and induction of apoptosis are associated with altered expression of the Bcl‐2 family of anti‐ and proapoptotic proteins. These differentiated HL‐60 cells are similar to mature human neutrophils, which do not express Bcl‐2 [12,13]. However, neutrophils do express the proapoptotic bax and bak at the mRNA
Discuss these hl-60 cells Lyrics with the community: We need you! Help build the largest human-edited lyrics collection on the web! Add Lyrics. The Web's Largest Resource for Music, Songs & Lyrics. A Member Of The STANDS4 Network. Search for song lyrics by Genres. The HL-60 cell line has proven to be a potent model for studies of human myeloid cell differentiation and differentiation in general. A 2015 article published in Environmental Toxicology (Chen et al.) investigates the cytotoxic effects of irinotecan HCl (CPT-11) in HL-60 cells in vitro and tumor development in the HL-60 leukemia xenograft model Transfection of HL-60 Cells Use this procedure to transfect plasmid DNA into HL-60 cells in a 24-well format (for other formats, see Scaling Up or Down Transfections, below). All amounts and volumes are given on a per well basis. The day of transfection, count the cells to determine culture density. Human leukemia HL-60 cells were gifted from the Institute of Hematology and Blood Diseases Hospital, Chinese Academy of Medical Sciences, Tianjin. HL-60 cells were cultured in RPMI-1640 medium (Gibco) supplemented with 10% heat-inactivated fetal bovine serum (FBS) in a … Abstract Two non-psychotropic cannabinoids, cannabidiol (CBD) and cannabidiol-dimethylheptyl (CBD-DMH), induced apoptosis in a human acute myeloid leukemia (AML) HL-60 cell line. Apoptosis was determined by staining with bisBenzimide and propidium iodide.
The HL-60 promyelocytic leukemia cell line: proliferation, differentiation, and cellular oncogene expression SJ Collins Molecular Medicine Program, Fred Hutchinson Cancer Research Center, Seattle, WA … Definition of hl-60 cells in the Definitions.Net dictionary. Meaning of hl-60 cells. What does hl-60 cells mean? Information and translations of hl-60 cells in the most comprehensive dictionary definitions resource on the web.
Differentiation of HL-60 to neutrophil or monocyte-like cells by Vitamin A (ATRA) and Vitamin D (1a,25(OH)2D3) Seed cells at 2.5x105 cells/ml as 4ml cultures in 25cm2 flasks. If seeding to plates, seed to base-density of approximately 1x106 cells/25cm2 e.G. 1cm2 well = 3.14cm2 25/3.14 = 8 1/8 = 0.125 x 10^6 cells… Human neutrophils and promyelocytic HL-60 cells bind specifically to COS cells transfected with GMP-140 complementary DNA and to microtitre wells coated with purified GMP-140.
HL-60 is a promyelocytic cell line derived by S.J. Collins, et al. Peripheral blood leukocytes were obtained by leukopheresis from a 36-year-old Caucasian female with acute promyelocytic leukemia.  HL-60 cells spontaneously differentiate and differentiation can be stimulated by butyrate, hypoxanthine, phorbol myristic acid (PMA, TPA In HL-60 cells incubated with 10 ng/ml of G-CSF only few aggregates of Qdots were localized in the cytoplasm and nucleus . In K562 control cells, the Qdots were observed mainly in the nucleus close to the heterochromatin . After treatment of K562 cells with 5 and 10 ng/ml of G-CSF nanocrystals were seen in both cytoplasm and nucleus. The secretory granule protein syncollin was first identified in the exocrine pancreas where a population of the protein is associated with the luminal surface of the zymogen granule membrane. In th... Bioalternatives present his test HL60-0001 : Macrophage-like HL-60 (vit. D3/TGF- beta-differentiated) : IL-8 release There are no reviews for HL-60 Lysate (NB800-PC3). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount. Review with no image -- $10/€7/ 1110 Yen Briefly, cells were administered with different concentrations of PTL and/or Ara-C. After incubation, the HL-60 cells were washed (230 g, 5 min), and then resuspended in 500 μL of PBS. Dead cells were stained with PI at a final concentration of 2 μg/mL and incubated for 15 min in the dark at room temperature. Cell culture.HL-60 cells were grown in a medium composed of Iscove's modified Dulbecco's medium, 20% fetal calf serum, and 0.5% gentamicin. The day before an experiment, cells were pelleted and resuspended in fresh medium at 10 6 cells per mL. The day of an experiment, 1 μmol/L PMA was added to the cells from a 1 mmol/L stock solution dissolved in dimethyl sulfoxide (DMSO). For the opsonophagocytic assay with HL-60 cells, bacterial strains were grown in 50 ml of Todd-Hewitt broth supplemented with 0.5% yeast extract to an optical density at 420 nm of 0.5 to 0.6 (mid-logarithmic growth phase for the majority of strains tested by using a 13-mm [outer diameter] path HL-60 cells have no detectable expression of HLA-DR-associated determinants as assayed by rabbit anti-p28,33 monoclonal anti-HLA-DR (monomorphic determinant), and HLA-DRw typing alloantisera. The relationship of these changes in cell surface properties to normal granulocytic differentiation is … HL-60 (promyeloblast; acute promyelocytic leukemia) Human HL-60 cells were cultured in Iscove's modified Dulbecco's medium with 4 mM L-glutamine and harvested at the log phase of growth. The HL-60 cells were fixed in 4% paraformaldehyde and arrayed on a 12-well (5 mm) adhesive coated slide, with each wellis surface specifically treated to enhance cellular attachment and to minimize background … Hl-60 cells lack specific markers for lymphoid cells but express surface receptors for fc fragment and complement. They also exhibit phagocytic activity and responsiveness to chemotactic stimuli . (20 … We set out to identify whether HOCl, which is generated from H 2 O 2 /MPO/Cl-, is a proximal mediator of H 2 O 2 programmed cell death in the HL-60 human leukemia cell. We found that authentic HOCl induces apoptosis in the HL-60 cell, Both the addition of methionine, an HOCl scavenger, and the removal of Cl-from the medium to prevent the formation of HOCl inhibited H 2 O 2-induced apoptosis. Oxidative stress-mediated intrinsic apoptosis in human promyelocytic leukemia HL-60 cells induced by organic arsenicals. Scientific Reports 2016, 6 (1) DOI: 10.1038/srep29865. Dongzhu Duan, Junmin Zhang, Juan Yao, Yaping Liu, Jianguo Fang.
ATRA induced differentiation of HL-60 cells along the granulocytic pathway within 48 h. The half-lives of bcl-2 mRNA in HL-60 cells incubated with ATRA for 48 or 72 h were reduced to 39 and 7% of the corresponding untreated control values, respectively. Human leukemia HL-60 cells from a 36-year-old female (JCRB0085) were purchased by Japan Health Sciences Foundation (Tokyo, Japan) and were cultured in Roswell Park Memorial Institute (RPMI) 1640 medium supplemented with 10% inactivated fetal calf serum … HL‐60 cells synthesize a proteoglycan that is almost completely secreted when PMA is present. To examine the fate of [35 S]sulfate‐labeled proteoglycans in HL‐60 cells, a pulse (5 min) and chase (up to 1 h) labeling was performed with [35 S]sulfate in the absence and presence of 50 nM PMA. Polypeptides from corresponding aliquots of cell ADCC. Unstimulated HL-60 cells possessed low levels of cytotoxicity to antibody-coated CE, with -10% specific cyto-toxicity at a ratio of 10 HL-60 cells to 1 CE (Fig. 3). Cells cultured for 5 dunderidentical conditions in the presence of y-IFNshowedathreetofourfold increaseinkilling. Thisdem-onstrates that recombinant human y-IFN is a potent